Peptides are short chains of amino acids (typically 2–50 amino acids long) linked by peptide bonds. They act as signaling molecules in the body, influencing a wide range of biological processes including growth hormone release, tissue repair, inflammation, metabolism, and skin health. Unlike full proteins, their smaller size allows them to be absorbed and utilized efficiently.

What is peptide reconstitution and why does it matter?

Reconstitution is the process of adding bacteriostatic water (sterile water with 0.9% benzyl alcohol) to a lyophilized (freeze-dried) peptide vial to create an injectable solution. The amount of water you add determines the concentration, which directly affects how much liquid you need to draw per dose. Always swirl gently — never shake — to avoid damaging the peptide bonds.

How should peptides be stored?

Unreconstituted (powder) peptides should be stored in a freezer or refrigerator away from light and moisture. Once reconstituted with bacteriostatic water, vials should be refrigerated at 36–46°F (2–8°C) and are typically stable for 4–6 weeks. Never freeze reconstituted peptides, and always keep them away from heat and direct sunlight.

What type of syringe should I use for peptides?

Insulin syringes are the standard for subcutaneous peptide administration. They come in three common sizes: 100-unit (1 mL), 50-unit (0.5 mL), and 30-unit (0.3 mL). Smaller syringes provide more precise measurements for small doses. For most peptide protocols, a 100-unit or 50-unit syringe with a 29–31 gauge needle is standard.

How do you calculate peptide dosing after reconstitution?

To calculate peptide dosing: First, convert the vial size to mcg (mg × 1000). Then divide total mcg by the mL of bacteriostatic water added to get concentration (mcg/mL). Divide your desired dose in mcg by the concentration to get the mL to inject. Multiply the mL by the syringe unit count (e.g. 100 for a 1mL syringe) to get the number of units to draw on your insulin syringe.

What is BPC-157 and what is it used for?

BPC-157 (Body Protection Compound-157) is a 15-amino acid peptide derived from gastric juice. It has been extensively studied for its ability to accelerate tissue healing across tendons, ligaments, muscle, and the gut lining. Research suggests it promotes angiogenesis, upregulates growth factor expression including VEGF, and has cytoprotective effects on the GI tract. Common research dosing ranges from 250 to 500 mcg administered once or twice daily.

What is Tesamorelin and how does it work?

Tesamorelin is an FDA-approved synthetic growth hormone-releasing hormone (GHRH) analog that stimulates natural growth hormone production from the pituitary gland. Unlike exogenous GH, it maintains the body's natural pulsatile release pattern. It has been clinically studied for reduction of visceral adipose tissue, improved body composition, and enhancement of IGF-1 levels. Standard clinical dosing is 1–2 mg administered subcutaneously once daily.

What is the difference between Sermorelin and Tesamorelin?

Both are GHRH analogs that stimulate natural growth hormone release, but they differ in structure and potency. Sermorelin consists of the first 29 amino acids of GHRH and was previously FDA-approved for GH deficiency in children. Tesamorelin is a modified GHRH analog that is currently FDA-approved for visceral fat reduction. Tesamorelin is generally considered more potent, while Sermorelin is often used as a more accessible entry-level GH secretagogue. Dosing also differs: Sermorelin at 100–300 mcg daily vs Tesamorelin at 1–2 mg daily.

TheDoseGuide — Peptide Dosing Calculator, Guides & Education for BPC-157, Tesamorelin, Sermorelin

Latest Research

Featured Articles

In-depth breakdowns, comparisons, and guides for the peptide research community.

🛡️

February 23, 2026

GLP-3 (Retatrutide) and Cancer: How a Weight Loss Peptide May Stop Tumor Growth

Preclinical research shows retatrutide (GLP-3) reduced tumor volume by up to 17-fold in cancer models. How the triple agonist fights tumors through immune reprogramming, YAP degradation, and metabolic correction.

Read Article →

💪

February 23, 2026

MOTS-c and Myostatin: How a Mitochondrial Peptide May Unlock Muscle Growth and Reverse Aging

Research shows MOTS-c reduced myostatin by 40% in mice, mimics exercise at the cellular level, activates AMPK, and reverses age-related muscle decline. The complete science-backed breakdown.

Read Article →

🧬

February 21, 2026

Copper Peptide (GHK-Cu) for Hair Growth: Complete Research Guide

How GHK-Cu stimulates hair follicles through Wnt pathway activation, angiogenesis, and anti-inflammatory effects. Includes comparison with Minoxidil and Finasteride, dosing protocols, and application methods.

Read Article →

⚔️

February 10, 2026

BPC-157 vs. TB-500: Understanding the Differences for Recovery

Both peptides are studied for tissue repair, but they work through different mechanisms. Here's what the research says about each and when one might be preferred.

Read Article →

🧪

February 5, 2026