How It Works
NAD+ is a critical cofactor for sirtuins (SIRT1-7), PARPs, and CD38 — enzymes involved in DNA repair, gene expression regulation, cellular stress response, and circadian rhythm maintenance. Subcutaneous NAD+ bypasses the digestive system for more direct cellular uptake compared to oral precursors like NMN or NR.
Reconstitution Guide
Follow these steps to properly reconstitute NAD+ for research use:
- Step 1: Swab the tops of both the peptide vial and bacteriostatic water vial with alcohol.
- Step 2: Draw your desired amount of BAC water (commonly 1–2 mL) into an insulin syringe.
- Step 3: Inject the water slowly into the peptide vial, aiming at the glass wall — not directly onto the powder.
- Step 4: Swirl the vial gently until fully dissolved — never shake.
- Step 5: Refrigerate at 36–46°F (2–8°C). Use within 4–6 weeks.
Use the dosing calculator to calculate your exact concentration (mcg per unit) and how many units to draw on your syringe.
Frequently Asked Questions
How does NAD+ work? ▾
NAD+ is a critical cofactor for sirtuins (SIRT1-7), PARPs, and CD38 — enzymes involved in DNA repair, gene expression regulation, cellular stress response, and circadian rhythm maintenance. Subcutaneous NAD+ bypasses the digestive system for more direct cellular uptake compared to oral precursors like NMN or NR.
What are the key research findings? ▾
- Essential cofactor for sirtuin activation and DNA repair pathways
- Research links NAD+ decline to aging, neurodegeneration, and metabolic disease
- Studies show improved mitochondrial function and energy metabolism
- Injectable route provides superior bioavailability vs. oral supplementation
What is the typical dosing protocol? ▾
Subcutaneous injection: beginners start at 50–100 mg daily. Standard protocols use 100–200 mg daily. The 1000 mg vial supports extended research. Some protocols use 2–3 times per week rather than daily. Note: SubQ injections may produce a temporary stinging sensation. Cycles can run ongoing with periodic blood work.
How should NAD+ be stored? ▾
Unreconstituted powder should be stored in a freezer or refrigerator away from light and moisture. Once reconstituted with bacteriostatic water, refrigerate at 36–46°F (2–8°C) and use within 4–6 weeks. Never freeze reconstituted peptides.